FAST SCAN CYCLIC VOLTAMMETRY SYSTEM

Pinnacle's robust, turn-key FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM is designed to simplify the measurement of catecholamines (i.e., dopamine, norepinephrine, etc.) and other electroactive analytes such as adenosine. It functions by rapidly cycling a voltage across an implanted carbon fiber sensor and measuring the resultant current. Both the tethered system for mice and rats and the wireless system for rats have built-in support for controlling an external stimulus. The system ships with Pinnacle's FSCV software.

Preprogrammed and Custom Waveforms

The FSCV software offers preprogrammed waveforms associated with the commonly studied analytes: dopamine, norepinephrine, serotonin and adenosine. Custom waveforms can be easily uploaded as well, and stored in a drop-down menu for quick selection.

OVERVIEW
ELECTRODES
CALIBRATION
SOFTWARE

HOW IT WORKS

Changes in analyte concentration are detected by rapidly cycling a voltage across an implanted carbon fiber sensor and measuring the resultant current. Pinnacle's FSCV system can measure spontaneous sub-second neurotransmitter release while conducting detailed behavioral studies. Both the wireless and tethered systems scan up to 1,000 V/s in a user-selectable range spanning -0.6 to +1.5 V. All systems have built-in support for controlling an external stimulus.

Key Features

Voltage Span: -0.6 V to +1.5 V
Max scan rate: 1,000 V/s
Max sweeps/second: 10
Max points/sweep: 1,000

3-D heat map of reduction/oxidation measurement data

CARBON FIBER ELECTRODES

Pinnacle's FSCV system uses CARBON FIBER ELECTRODES (CFEs) to measure the presence of dopamine and other catecholamines in the brains of freely moving animals. All Pinnacle CFSs require an Ag/AgCl reference electrode.

CALIBRATION

In order to relate the current changes measured by a sensor to actual changes in concentration, it is necessary to calibrate the sensor at the conclusion of an experiment. This process also confirms the integrity and selectivity of the sensor.

CALIBRATION KITS
Product	Kit #
FSCV Calibration Kit	8500-K5

FSCV SOFTWARE PACKAGE

The FSCV system includes Pinnacle's FREE FSCV software, which supports traditional, short recording paradigms (recordings of two minutes or less) as well as longer-term recordings that use an extended continuous mode. Furthermore, the suite supports integrated, synchronized video recording, which allows monitoring of animal behavior simultaneously with biogenic amine release.

Features include:

• Background subtraction
• 3-D visualization
• User-selectable filters
• Heat maps
• Preprogrammed and custom waveforms
• Import/export to third-party packages

Analysis

A graph showing signature waveforms for dopamine, serotonin and norepinephrine. Dopamine (blue), serotonin (green), and norepinephrine (red) display specific voltammogram profiles when detected by FSCV.

Supporting Products

Synchronized Video

Integrated video recording makes it easy to correlate changes in analyte concentration with observable behavior. Add Pinnacle's Synchronized Video to any new or existing hardware system to simultaneously capture and display live video and synchronize it with other recorded data in playback mode.

Cages and Accessories

Circular cages and cage accessories are designed to allow rodents to freely move around a cage while connected to a tether. All cages are made of 1/4" clear acrylic and are suitable for use with most commercial cage washing equipment. Multiple sizes available.