SLEEP DEPRIVATION SYSTEM

Pinnacle's AUTOMATED SLEEP DEPRIVATION SYSTEM is designed for sleep deprivation and sleep fragmentation studies in mice and rats. The system enables precise, automated control of deprivation protocols without direct human intervention, while minimizing exercise-related confounds. The core system can be expanded with Pinnacle's FEEDBACK PRO SOFTWARE and EEG/EMG HARDWARE for closed-loop functionality. When equipped with Feedback, real-time EEG/EMG signals are used to determine sleep–wake state and automatically initiate deprivation as needed.

SYSTEM OVERVIEW
AVAILABLE FEATURES

HOW IT WORKS

PINNACLE'S SLEEP DEPRIVATION SYSTEM is designed to gently restrict sleep without unnecessary exercise in rodents. This is achieved by a rotating bar placed a short distance above the cage floor, lightly nudging the animal from sleep and encouraging low levels of activity until the animal maintains wakefulness on its own. Add Feedback software to enable advanced options and real-time control.

The system can be controlled by a computer or by the programmable interface on the front panel of the device.

The bar rotates at speeds between 1 to 12 RPM, operates at 3 dB or less, and supports programmable direction changes to reduce animal acclimatization.

The system accommodates water, food, and bedding. A variety of cage sizes are availble for mice and rats.

For advanced options and increased flexibility, see our Feedback Sleep Deprivation system.

SLEEP DEPRIVATION SYSTEM

The CORE SYSTEM can be programmed to operate on a user-defined temporal schedule. Upgrade by adding FEEDBACK PRO to receive a software license key that increases scheduling flexibility by incorporating real-time biopotential feedback, yoked control functionality, and more! Use the chart below to determine which pieces you need.

SYSTEM FEATURES	CORE SYSTEM	+ FEEDBACK
Available for both mice and rats
Adjustable speed and motor control
Calendar-based scheduling
Suitable for short-term, long-term, and chronic studies
Optional video recording
Compatible with third-party systems
Real-time biopotential analysis and feedback
Rule-based programming
Yoked control functionality
Requires Pinnacle's EEG/EMG system

KITS FOR MICE AND RATS

Pinnacle's stand alone sleep deprivation kits include the sleep deprivation unit, and a cage with water bottle. The system can be retrofitted for the automatic feedback feature.

HARDWARE KIT
Product		Kit #
Stand Alone Sleep Deprivation Kit for Mice (10" cage)		9000-K5-S
Stand Alone Sleep Deprivation Kit for Rats (12" cage)		9000-K6-S
Stand Alone Sleep Deprivation Kit for Rats (14" cage)		9000-K6-S-14

SLEEP DEPRIVATION EFFECTIVENESS IN MICE

The chart to the right shows a comparison of baseline sleep (purple bars) with a 24-hour sleep deprivation period (gold bars). Six mice (male, C57BL/6J) were maintained on a 12-hour lights-on (horizontal tan)/ 12-hour lights-off (horizontal black) schedule. EEG and EMG activity were recorded for 24 hours (baseline). During the following 24 hours, each animal was sleep deprived by programming the system to rotate the bar within ten seconds of the mouse entering a sleep-like state, as determined by active EEG feedback. Vertical bars represent sleep time in 2-hour bins.

A Pinnacle EEG/EMG system is required to operate the sleep deprivation unit in feedback mode. Pinnacle offers three- and four-channel systems for biopotential recording. Tethered solutions are available for both mice and rats.

Synchronized video recording can be added to any new or existing Pinnacle system, providing a platform for correlating changes in brain chemistry with behavioral states. Captured video can be displayed live on screen during acquisition, and, in playback mode, video is synchronized with recorded data.

PINNACLE'S SIRENIA^® SLEEP PRO software allows users to reduce scoring and analysis time by automating the process with tools such as cluster scoring, threshold scoring, hypnograms, and spectral plots. The software’s analysis features allow users to quickly compare scores, perform bout and sleep analyses, and customize high-quality charts and graphs.