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Expanded Optogenetics Line

Nine headstages are now available for optogenetic studies in either tethered mice or rats. Most combinations of sleep, seizure, biosensor and optical stimulation can now be easily studied. Seven different wavelength LED probes are available which are compatible with most opsins.

To show you an example of the power of the system, we used a kindling model as part of our validation studies to generate seizures in a transgenic mouse [( Thy1-COP4/EYFP)9Gfng] expressing channelrhodopsin from Jackson Laboratory. The figure below shows a resultant seizure along with changes in glutamate.

EEG of Kindling seizure induced by optogenetic stimulation

(Click image to enlarge)

Cortical EEG and hippocampal depth electrode activity was monitored along with prefrontal cortex glutamate changes. A 445 nm blue LED fiber probe was used for stimulation with light pulses at 20 Hz, 10% duty cycle, 600 mA, delivered for 20 seconds. Six stimulations occurred per day (30 minutes apart over a three-hour period). Initial LED power was 130 mW/mm².

Headstage kits available:

Headstage Kits Mouse Kit # Rat Kit #
1 or 2 Opto
1 Opto, 1 Biosensor
1 Opto, 3 EEG Seizure
1 Opto, 3 EEG, 1 Bio Seizure
1 Opto, 4 EEG Seizure
1 Opto, 2 EEG, 1 EMG Sleep
1 Opto, 2 EEG, 1 EMG, 1 Bio Sleep
2 Opto, 3 EEG Seizure
2 Opto, 2 EEG, 1 EMG Sleep

LED probes available:

LED Fiber Probes Power (mW/mm²)
Mouse
Item #
Rat Locking Item # Rat Friction Fit Item # Peak Wavelength (nm)
@300 mA @600 mA
445 Deep Blue   69 114
465 Blue   66 112
515 Green   62 NR*
590 Yellow   78 NR*
620 Amber   87 162
640 Red   82 104
660 Deep Red   97 163
Average power measured at the fiber probe tip. (1 kHz, 10% duty cycle). *Not recommended.
To learn more about this system, visit our poster at SFN (92.29/KKK27) on Saturday afternoon or stop by our booth #2721.